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牛转化生长因子α(TGFa)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Cattle Transfor Cattle ming Growth Factor Alpha (TGFa)
别名: ETGF; TGF-A; Protransforming Growth Factor alpha; EGF-Like TGF 品牌 :USCN/优尔生-A 商品货号 : USEA123Bo 存储条件 : 4℃
规格 类别 价格 货期
96T A 1701.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Alpha (TGFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Alpha (TGFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Alpha (TGFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Alpha (TGFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Transforming-Growth-Factor-Alpha-(TGFa)-SEA123Bo.htm