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大鼠铜伴侣超氧化物歧化酶检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Rat Superoxide Dismutase Copper Chaperone
别名: CCS; Copper Chaperone For Superoxide Dismutase 品牌 :USCN/优尔生-A 商品货号 : USEA596Ra 存储条件 : 4℃
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96T A 1539.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase Copper Chaperone. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase Copper Chaperone. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase Copper Chaperone, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutase Copper Chaperone in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Superoxide-Dismutase--Copper-Chaperone-(SOD4)-6937.htm