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牛山梨醇脱氢酶(SDH)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Cattle Sorbitol Dehydrogenase (SDH)
别名: SORD; L-iditol 2-dehydrogenase 品牌 :USCN/优尔生-A 商品货号 : USEB495Bo 存储条件 : 4℃
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96T A 1795.50 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorbitol Dehydrogenase (SDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorbitol Dehydrogenase (SDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorbitol Dehydrogenase (SDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sorbitol Dehydrogenase (SDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Bovine-Sorbitol-Dehydrogenase-(SDH)-6635.htm