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人拓扑异构酶Ⅰ(TOP1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Human Topoisomerase I (TOP1)
别名： TOPI; DNA Topoisomerase 1
品牌 ：USCN/优尔生-A
商品货号 ： USEC243Hu
存储条件 ： 4℃
| 规格 | 类别 | 价格 | 货期 | |
| 96T | A | 1575.00 | 3天 |
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产品特点：
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Topoisomerase I (TOP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Topoisomerase I (TOP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Topoisomerase I (TOP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Topoisomerase I (TOP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Human-Topoisomerase-I(TOP1)-4691.htm
