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人N-乙基马来酰亚胺敏感因子(NSF)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Human N-Ethylmaleimide Sensitive Factor (NSF)
别名: SKD2; N-Ethylmaleimide Sensitive Fusion Protein; Vesicle-fusing ATPase; Vesicular-fusion protein NSF 品牌 :USCN/优尔生-A 商品货号 : USEC648Hu 存储条件 : 4℃
规格 类别 价格 货期
96T A 1575.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Ethylmaleimide Sensitive Factor (NSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Ethylmaleimide Sensitive Factor (NSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-N-Ethylmaleimide-Sensitive-Factor-(NSF)-30916.htm