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大鼠丙酮酸脱氢酶激酶同工酶1(PDK1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Rat Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)
别名: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial 品牌 :USCN/优尔生-A 商品货号 : USEC718Ra 存储条件 : 4℃
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96T A 1710.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Pyruvate-Dehydrogenase-Kinase-Isozyme-1-(PDK1)-30918.htm