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大鼠Ⅱ型胶原α1(COL2a1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Rat Collagen Type II Alpha 1 (COL2a1)
别名: COL2-A1; COL2A1; SEDC; Chondrocalcin; Collagen Alpha-1(II)Chain; Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital 品牌 :USCN/优尔生-A 商品货号 : USED194Ra 存储条件 : 4℃
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96T A 1710.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type II Alpha 1 (COL2a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type II Alpha 1 (COL2a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type II Alpha 1 (COL2a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type II Alpha 1 (COL2a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Collagen-Type-II-Alpha-1-(COL2a1)-8463.htm