大鼠脯氨酸-4-羟化酶α多肽Ⅰ(P4Ha1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Rat Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1)
别名: Procollagen-Proline,2-Oxoglutarate 4-Dioxygenase(Proline 4-Hydroxylase),Alpha Polypeptide I
品牌 :USCN/优尔生-A
商品货号 : USEE253Ra
存储条件 : 4℃
| 规格 | 类别 | 价格 | 货期 | |
| 96T | A | 1710.00 | 3天 |
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产品特点:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Rat-Prolyl-4-Hydroxylase--Alpha-Polypeptide-I-(P4Ha1)-6213.htm

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