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大鼠N-酰基鞘氨醇酰胺水解酶2(ASAH2)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Rat N-Acylsphingosine Amidohydrolase 2 (ASAH2)
别名: HNAC1; non-lysosomal ceramidase; Neutral ceramidase 品牌 :USCN/优尔生-A 商品货号 : USEE689Ra 存储条件 : 4℃
规格 类别 价格 货期
96T A 1710.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acylsphingosine Amidohydrolase 2 (ASAH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Acylsphingosine Amidohydrolase 2 (ASAH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acylsphingosine Amidohydrolase 2 (ASAH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Acylsphingosine Amidohydrolase 2 (ASAH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-N-Acylsphingosine-Amidohydrolase-2-(ASAH2)-SEE689Ra.htm