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人甘露糖磷酸异构酶(MPI)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Human Mannose Phosphate Isomerase (MPI)
别名: PMI1; Mannose-6-Phosphate Isomerase; Phosphohexomutase; Phosphomannose isomerase 品牌 :USCN/优尔生-A 商品货号 : USEH620Hu 存储条件 : 4℃
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96T A 1575.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Phosphate Isomerase (MPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Phosphate Isomerase (MPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Phosphate Isomerase (MPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Phosphate Isomerase (MPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Mannose-Phosphate-Isomerase-(MPI)-28079.htm