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大鼠自噬相关蛋白5(ATG5)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Rat Autophagy Related Protein 5 (ATG5)
别名: ASP; APG5; APG5L; hAPG5; Apoptosis-specific protein; Autophagy protein 5 品牌 :USCN/优尔生-A 商品货号 : USEL221Ra 存储条件 : 4℃
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96T A 1881.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 5 (ATG5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 5 (ATG5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 5 (ATG5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autophagy Related Protein 5 (ATG5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Autophagy-Related-Protein-5-(ATG5)-SEL221Ra.htm